I'm sorry, but I missed the original thread. Did someone suggest or find
that GFP synthesized at < 37C doesn't flouresce?
I've been working with GFP and zebrafish, which must be raised around
28C, so I'm wondering whether this may be a source of my problems.
If GFP must be synthesized @ 37C, how does the jelly-fish from which the
protein was originally from, produce it in the sea if it's ectothermic?
ylin at acpub.duke.edu
On 15 Jan 1996, Masaru Okabe wrote:
> At 4:02 PM 1/15/96 +0000, David de Graaf wrote:
>> de Graaf> but GFP
> de Graaf>does not fluoresce when it is synthesized at 37.
>> In transgenic mice, GFP (and RS-GFP) is produce in the environment of 37 C
> or higher and fluorecent.
>> Masaru Okabe