This response seems similar to the experience that I have
Mike Moser Tel: 206-543-5354
UW Department of Biochemistry FAX: 206-685-1792
Box 357350 moser at u.washington.edu
Seattle, WA 98195-7350 Make peace my beast is yeast
On Tue, 2 Jan 1996, Brandt Cassidy wrote:
> In article <4c1fv5$jrm at itssrv1.ucsf.edu>, Brian Wickes
> <wickes at cgl.ucsf.edu> wrote:
>> > Has anyone had difficulty expressing GFP-1 in bluscript? How did you
> > make your construct (using SK+ and GFP from pGFP-1) and was a longwave
> > hand held UV light ok?
> > Brian
>> We found that only some of the colonies from the transformation gave very
> high fluorescence. We did use a hand held UV light source to detect the
> Fluorescence. It helps to have a dark room to see it. A nontransformed E.
> coli control is good for comparison as well.
>> Brandt G. Cassidy
> Assistant Scientist
> Plant Biology
> The Samuel Roberts Noble Foundation
> e-mail BGCASSIDY@ noble.org