In article <3qkcda$4p6 at netnews.upenn.edu>,
Edward Zartler <zartler at a.chem.upenn.edu> wrote:
>> My question is simple, we came upon this protein in looking at other
>stuff, and we had an idea can any body help me.
>> The chromphore is formed by the SYG at 64-66 (?). How often does this
>sequence occur in other proteins??? Is the presence of this sequence enough
>to generate the chromphore and if it isn't how much of the surrounding protein
>do you need.
>> I say all, Ponzy (my boss) says find out...
>> Any help is appreciated.
>I believe the evidence for GFP itself in E. coli is that almost all of
the sequence is necessary for fluorescence. Only a few amino acids can
be deleted from either end without losing activity. Also Roger Tsien's
group has shown an effect of a mutation 100 amino acids away from the
William J. Buikema Phone: (312)702-1081 e-mail: hetman at uchicago.edu
Dept. Mol. Genet. & Cell Biol. / / --- --- /|/| /| /| /
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