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[Drosophila] New Methods article - imaging live muscle in prepupae

Roy Edward via dros%40net.bio.net (by roy from biostatus.com)
Tue Oct 13 11:39:49 EST 2015

The thermo-reversible hydrogel mountant CyGEL is described for the live imaging of muscle in Drosophila prepupae. 

Puah, Wee Choo, and Martin Wasser. "Live Imaging of Muscles in Drosophila Metamorphosis: Towards high-throughput gene identification and function analysis." Methods (2015).

Objects can be recovered gently for onward growth or orthogonal analysis and avoids risk of hyperthermal shock from use of LMP agarose.

View an example of time-lapse imaging of a larva here: 

Technical details available here:

Review article on CyGEL:
Edward, R. American Biotechnology Laboratory. July/August 2010 Issue .. 

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