We annotate using the genome sequence. Coincidentally, we just published a pair of papers concerning our annotation process in G3.
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Sian Gramates, FlyBase
On Jul 7, 2015, at 4:56 AM, Sina Wäldchen <sina.waeldchen from uni-wuerzburg.de> wrote:
>> I am really new to flybase and I have a short question. I am searching for a mRNA sequence to design complementary oligonucleotides for FISH. Is the FastA that I get if I choose "Transcripts" the real mRNA sequence or is it the belonging complementary DNA sequence? So, do I have to use the complementary sequence to get my mRNA sequence or can I use it directly?
>> Kind regards,
> Sina Wäldchen
> Lehrstuhl für Biotechnologie und Biophysik
> Universität Würzburg
> Biozentrum - Am Hubland
> D-97074 Würzburg
> +49 931 31-84581
>sina.waeldchen from uni-wuerzburg.de>> _______________________________________________
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