IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Drosophila] Regarding Drosophila sperm tail staining

Khire, Atul D via dros%40net.bio.net (by Atul.Khire from rockets.utoledo.edu)
Mon Jun 24 07:33:18 EST 2013



Hello,
This is regarding my query about sperm tail staining. I tried alpha tubulin and its not working.I have ordered donJoun GFP fly from bloomington.I also went through the literature and most people have used AX-D5 antibody for the sperm tail staining.Does any one have AX-D5 antibody to spare a small aliquot for me?
Thanks a lot,
Sincerely,
Atul D.Khire
PhD student,
Prof.Tomer Avidor-Reiss Lab,
Department of Biological Sciences,
University of Toledo,
Toledo,Ohio
USA

________________________________________
From: Khire, Atul D
Sent: Friday, June 14, 2013 8:57 AM
To: Maria Enrica Pasini
Subject: Re: [Drosophila] Regarding sperm tail staining

Dear Dr.Pasini
Thanks a lot for your prompt reply.I would definitely implement your advice
Sincerely
Atul

Sent from my iPhone

On Jun 14, 2013, at 8:23, "Maria Enrica Pasini" <maria.pasini from unimi.it> wrote:

> Lecitina is a mistake of my ipad, lectins WGA or GNA is correct.
> Maria
>
>
>
> Il 14/06/13 13:28, Khire, Atul D ha scritto:
>> Dear Dr.Pasini,
>> Thanks a lot for your very helpful advice.I have tried acytylated tubulin but that is not working.Now I will stain with antibodies suggested by you.
>> Sincerely,
>> Atul D.Khire
>> PhD student,
>> Prof.Tomer Avidor-Reiss Lab,
>> Department of Biological Sciences,
>> University of Toledo,
>> Toledo,Ohio
>> USA
>>
>> ________________________________________
>> From: Maria Enrica Pasini [maria.pasini from unimi.it]
>> Sent: Friday, June 14, 2013 1:55 AM
>> To: Khire, Atul D
>> Subject: Re: [Drosophila] Regarding sperm tail staining
>>
>> Lecitina or anti- Hexo mark tail surface or anti-alfa tubulin
>>
>>
>> Maria Enrica Pasini
>> Dipartimento di Bioscienze
>> Università' degli Studi di Milano
>> Via Celoria 26, 2B
>> 20133 Milano
>> Tel. 02 50314887
>> Fax 02 50314880
>> e-mail: maria.pasini from unimi.it
>>
>> Il giorno 13/giu/2013, alle ore 22:31, "Khire, Atul D"<Atul.Khire from rockets.utoledo.edu>  ha scritto:
>>
>>> Hello,
>>> Would anyone please tell me a good antibody to stain the sperm tail of drosophila?
>>> Thanks
>>>
>>>
>>> Atul D.Khire
>>> PhD student,
>>> Prof.Tomer Avidor-Reiss Lab,
>>> Department of Biological Sciences,
>>> University of Toledo,
>>> Toledo,Ohio
>>> USA
>>> _______________________________________________
>>> Dros mailing list
>>> Dros from net.bio.net
>>> http://www.bio.net/biomail/listinfo/dros
>
>
> --
> Maria Enrica Pasini
> Dipartimento di Bioscienze
> Università degli Studi di Milano
> via Celoria 26, 2B
> I-20133 Milano
> Tel: +39 02 5031 4887
> Fax: +39 02 5031 4881
> E-mail: maria.pasini from unimi.it
>
>




More information about the Dros mailing list

Send comments to us at biosci-help [At] net.bio.net