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[Drosophila] overseeding

Nigel Atkinson via dros%40net.bio.net (by NigelA from mail.utexas.edu)
Thu Nov 15 11:54:00 EST 2012

Too many flies is a bad idea.
I like 10 females and 10 males per bottle. I sometimes use the same amount
for vials. Works well for both.


On Wed, Nov 14, 2012 at 6:55 PM, Mark Thorson <nospam from sonic.net> wrote:

> I've noticed some of my cultures have a syndrome
> I think is caused by seeding with too many adults.
> The symptoms are many larvae, few pupae, and few
> adults; unusually flat surface to the culture medium
> aside from sort of a pebble grain texture; and most
> of all, when light reflects off the surface of the
> culture medium I can see the whole thing is alive
> with motion.  It's that last one which makes me
> suspect these cultures are suffering from larval
> crowding.  Does that sound right, and if so, is
> overseeding the cause?
> For the past few days I've been careful to limit
> the number of adults I use to about 20-25 per
> culture.  I don't know what the optimal number is
> for my culture system, but from experiments I did
> several months ago I know there is no benefit from
> using more than 30.  I have probably been using
> closer to 50, and I think the improvement in my
> methods after adding calcium propionate has brought
> out this new failure mode.  It'll be a week or two
> before I know whether reducing the number of adults
> used to start a culture eliminates the problem, but
> even then I might not be sure because it is a
> sporadic problem -- usually only a few cultures
> are affected, though I did have one batch in which
> all the cultures were affected.
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