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[Drosophila] PhiC31 - PBac{y[+]-attP-9A} lines

Viet Le via dros%40net.bio.net (by viet_le from brown.edu)
Thu Feb 3 22:20:45 EST 2011

Hi all-

I'm using PhiC31 for targeted integration of genomic rescue constructs and
reporter constructs and would like to know if anyone has any experience
using the following PBac{y[+]-attP-9A} lines:

     Bloomington Stock # Symbol Cytology RTF order number
PBac{y[+]-attP-3B}VK00008 <http://flybase.org/reports/FBti0076431.html> 50A3
9728 <http://flystocks.bio.indiana.edu/Reports/9728.html>
PBac{y[+]-attP-9A}VK00030 <http://flybase.org/reports/FBti0076450.html>
5.00E+02 9747 <http://flystocks.bio.indiana.edu/Reports/9747.html>
PBac{y[+]-attP-9A}VK00018 <http://flybase.org/reports/FBti0076439.html> 53B2
9736 <http://flystocks.bio.indiana.edu/Reports/9736.html>
PBac{y[+]-attP-9A}VK00022 <http://flybase.org/reports/FBti0076443.html> 57F5
9740 <http://flystocks.bio.indiana.edu/Reports/9740.html>

Specifically, I am concerned about the level of (trans)gene expression at
these sites.  Any information would be greatly appreciated.

Thank you.

Viet Le
Ph.D. Candidate
Wharton Laboratory
Department of Molecular Biology, Cell Biology, and Biochemistry
Box G-L373
Brown University
Providence, RI 02912
viet_le from brown.edu

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