My dear fly colleagues,
I've recently got some flies from exilixis collectiom at Harvard
But, I am confused with these because I am finding dominant markers
such as stubble that are not shown in Flybase genotype info. in most
of the lines that i obtained for my gene. Does this mean the insertion
is heterozygous? on third as my gene is on third chromosome.
Once I obtain the lines shoud I outcross (to cantonise) for example
to mitigate position effects in case if i want to qPCR compare the
gene expression with a matched control fly like w-.
Please somebody help me.
Many thanks in advance.