I am facing problems with homogenous immunostaining of the whole Dosophila
larva (3rd instar) brain, as I found after confocal microscopy that the
staining / antibody penetration into the interior of the brain was poor. May
I know what are some of the solutions to overcome this problem and which
holder device should I perform this immunostaining experiment in so as to
obtain good mixing during the immunostaining process and do not have to
worry about the loss of the very small-sized larvae brains?
I would greatly appreciate any suggestions given. Thank you very much for
your kind help.
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