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[Drosophila] protein expression

Nabanita Chatterjee via dros%40net.bio.net (by chatterjee.n from gmail.com)
Fri Feb 6 10:35:15 EST 2009


I am trying to express a putative 12 transmembrane neurotransmitter
transporter like drosophila protein in bacterial cells, for future antibody
production,can anybody help me with choosing a correct vector?
I tried with pet28b+ ,my BL21 cells are dying.
also I would like some advice as how to tag my protein with GFP/RFP.

Many thanks

"To live and not to know why the cranes fly,why children are born,why the
stars are in the sky.Either you know why you're alive or it's all
nonsense,it's all dust in the wind"

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