Hello Drosophila Community,
I'm looking for a good protocol, or even just general tips for making protein extracts from third-instar larvae and adult flies. I'm dealing with a very large protein, and I'm having a lot of trouble avoiding degradation. Grinding adults/larvae/salivary glands directly in boiling SDS loading buffer hasn't worked, nor has one protocol where I included protease inhibitors at every step. Any advice at all would be greatly appreciated!