I saw your post on your sick flies. I don't know if this will help or not,
but we use molasses instead of sucrose, etc. Here is our recipe:
400ml h2o (filtered/deionized)
but for our antibiotic we are using 5.3mL tegostat (.53g/g.3mL 95% EtOH) and
1.8 mL propionic acid.
There are several reasons that I can think of that your colony might be not
happy, but none are conclusive:) They might be sensitive to your
antibiotic, they might not like the pH of the sugars, there might be
something in the water supply. I would start by swapping things out of your
recipe, and see if you can locate the source. I would start with the water,
and then the antibiotic.
Hope that helps! -isabelle
isabelle from ucsc.edu is not reliable
Please use ismirth from gmail.com instead.
"A person who says 'it can't be done' shouldn't be interrupting the person