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[Drosophila] Drosophila pupal epithelium

Pedro Alex via dros%40net.bio.net (by ppinto from ibmc.up.pt)
Mon Mar 5 07:10:59 EST 2007

Hi  Marc,

I am working a little bit with drosophila epithelium, specifically the
abdomen. What I can say is that it is not very difficult to dissect
Drosophila pupae (at leats with 26h APF). There is a protocol in the web
from a Nicolas Gompel that is very good. 
Good Luck


Marco Antunes wrote:
> Hello!
> I'm interested in working in the Drosophila pupal epithelium.
> However, most literature about Drosophila pupa is very old...
> Does anyone have some ideas about the difficulties and protocols for
> dissecting and manipulating Drosophila pupae (without getting it killed)?
> Also, does anyone know which is the best part of the pupa to visualize the
> epithelium? The Thorax or the Abdomen?
> Thank you for any help!
> Marc
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