There is a table in "Biology of Drosophila" (edited by M. Demerec) that
gives hours after egg laying (AEL) for larval stages at 25 degrees C,
plus or minus 1 degree. If you are doing your experiments at 23 degrees,
you might have to increase the time slightly. Here is the relevant data
from the table:
1st instar: 25h AEL
2nd instar: 50h AE
2nd and 3rd instar: 75h AEL
3rd instar, ready for pupation: 100h AEL
Jack Gaynor wrote:
>> I have been unable to locate the number of hours for the larval
> stages. We have been setting up learning assays with 3rd instar
> larva and have been harvesting these at 100 hrs after egg laying.
> However, we are now trying to do the same for 1st and 2nd instar
> stages and are not sure exactly when to harvest them. I realize this
> must be well know, but have not been able to locate the data. We are
> culturing at 23C.
>> Also, we have experimented with both Canton-S and Oregon R wild
> type. I expected these to be very similar, but we have seen some
> significant differences in the response of 3rd instar larvae to light
> in these training assays. Since we are relatively new to fly work,
> can anyone give us a sense of how similar or different these two wt
> strains are? Which is the more "acceptable" line to work with?
>> Many thanks!
>> Jack Gaynor
>>> John J. Gaynor, Ph.D.
> Department of Biology & Molecular Biology
> Science Hall, SH114
> Montclair State University
> Montclair, NJ 07043
>> voice: 973-655-7253
> fax: 973-655-7047
> lab: 973-655-4075
> email: jack.gaynor from montclair.edu>> _______________________________________________
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