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[Drosophila] Larval Development

Sandra Zimmerman via dros%40net.bio.net (by szimmerm from andrew.cmu.edu)
Sun Jun 10 08:59:49 EST 2007

There is a table in "Biology of Drosophila" (edited by M. Demerec) that 
gives hours after egg laying (AEL) for larval stages at 25 degrees C, 
plus or minus 1 degree. If you are doing your experiments at 23 degrees, 
you might have to increase the time slightly. Here is the relevant data 
from the table:
1st instar: 25h AEL
2nd instar: 50h AE
2nd and 3rd instar: 75h AEL
3rd instar, ready for pupation: 100h AEL

Sandra  Zimmerman

Jack Gaynor wrote:

> Hi,
>     I have been unable to locate the number of hours for the larval  
> stages.  We have been setting up learning assays with 3rd instar  
> larva and have been harvesting these at 100 hrs after egg laying.   
> However, we are now trying to do the same for 1st and 2nd instar  
> stages and are not sure exactly when to harvest them.  I realize this  
> must be well know, but have not been able to locate the data.  We are  
> culturing at 23C.
>     Also, we have experimented with both Canton-S and Oregon R wild  
> type.  I expected these to be very similar, but we have seen some  
> significant differences in the response of 3rd instar larvae to light  
> in these training assays.  Since we are relatively new to fly work,  
> can anyone give us a sense of how similar or different these two wt  
> strains are?  Which is the more "acceptable" line to work with?
>     Many thanks!
> Jack Gaynor
> John J. Gaynor, Ph.D.
> Department of Biology & Molecular Biology
> Science Hall, SH114
> Montclair State University
> Montclair, NJ  07043
> voice: 973-655-7253
> fax: 973-655-7047
> lab: 973-655-4075
> email: jack.gaynor from montclair.edu
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