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[Drosophila] nuclei isolation

AJ Burdette via dros%40net.bio.net (by ajburdette from bama.ua.edu)
Sun Apr 22 22:14:45 EST 2007

I'm working on Drosophila S2 cells and am attempting to isolate 
purified nuclei from them.  Eventually I need to isolate the nuclear 
envelope intact.  I have been unable to find any papers online dealing 
with nuclei isolation from drosophila S2 cells and have been trying 
instead to piece together a protocol from papers dealing with other 
cell cultures.  Does anybody know of a protocol specifically for 
drosophila S2 nuclei isolation? Any information is greatly appreciated
AJ Burdette

The Caldwell Lab
The University of Alabama
Box 870344
411 Hackberry Ln
Tuscaloosa, AL 35487-0344

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