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[Drosophila] (no subject)

Kavita Vadali kvadali at ilstu.edu
Thu Feb 23 13:09:51 EST 2006

I am trying to do immunostaining of fly ovaries with anti-crumbs antibody
(ascites from DSHB) and am failing at it. I have tried 1:1000 and 1:500
concentrations of the antibody but I don't see even a faint signal. I want to
try methanol fixing the ovaries but I don't have the protocol for that. Has
anybody done this fixation on ovaries? Could you please send me the detailed
protocol for that?
Thanks a lot.

Kavita Vadali

Kavita Vadali
PhD Candidate
Division of Biomedical Sciences,
Department of Biological Sciences,
Illinois State University,
Campus Box 4120,
Normal, IL 61790-4120
Phone: (309)438-5075
Fax: (309)438-3722

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