Thank you to all of those who have replied thus far. Most of those who
have replied had questions so I'm going to clarify a few things:
I'm keeping W flies in standard temperature and humidity. The eggs
are being layed on molasses-agar laying plates. The difficulty doesn't
seem to be a result of the injecting process. Instead, the problem seems
to be that in the strain I have only a third of the eggs that are layed
actually hatch. This is before anything is done to them. Thus when I do
inject the flies, only a third (at most) of the flies injected can
theoretically survive, since only a third survive hatching to begin with.
I hope this helps. I'd really appreciate any suggestions you might have.
Thank you very much,
University of Richmond
Richmond, VA, USA
> Dear all,
>> In the strain of flies I have available to me only a third of the eggs
> that are layed actually hatch. This is really slowing down my injection
> experiment. Does anybody know what might be causing this and/or have any
> suggestions that might help increase the egg hatching rate of this strain?
>> Thank you very much,
> Kevin Miller