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[Drosophila] Re: Expression analysis by RT-PCR

Trav travis.johnson at sci.monash.edu.au
Sun Nov 20 22:21:05 EST 2005


I've recently been interested in the same Q. The answer depends upon
the treatment you intend to provide to the flies/cells. A good idea is
to try a few, firstly to see if your treatment alters their expression
(if you don't already know from microarray or previous work), and
secondly so that you have more options when matching efficiencies or
when you get a poor melt from a dodgy primer pair.

Trav



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