I am planning to use dsRNA to silence my gene of interest in
Drosophila S2 cell line. I am wondering which positive control should
I use. I dont have an S2 cell line having integration of luciferase
Also, I do have an antibody for my gene of interest, but would go for
first for RT-PCR as I dont have any other common antibody to see the
expression of a given positive control. By the way, I expect that
the dsRNA to my gene shall arrest the cell cycle.
Your suggestions and help will be appreciated.
Institute for Genetics