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website posting

Rebecca Petersen Brown rpbrown at uchicago.edu
Thu Oct 21 14:41:23 EST 2004

Please post on your website: 

I am trying to amplify 2 kb regions in the promoters of Drosophila genes and 
need a very high fidelity enzyme. I cannot tolerate any PCR errors. I have 
tried MasterAmp Extra Long Polymerase Mix from Epicentre Technologies but it 
is encorporates too many errors. I have been trouble shooting with 
Stratagene's PfuUltra Hot Start High Fidelity enzymes but I did not find 
appropriate sized bands even after many attempts to modify annealing 
temperature, and modifying amounts of DMSO, template DNA, salts, dNTPs, 
enzyme, or primers. Does anyone recommend a very high-fidelity enzyme for 
amplification of 2kb from Drosophila genomic DNA? 

Becca Brown 
rpbrown at uchicago.edu 

Rebecca Petersen Brown
NSF Postdoctoral Fellow
The University of Chicago
Department of Organismal Biology & Anatomy
1027 East 57th Street
Chicago, IL 60637-1508
tel. 773-834-0467
fax. 773-702-0037
email rpbrown at uchicago.edu

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