I am using flies which stably express a long inverted repeat for RNAi of a
specific gene from a UAS regulatory element. I have obtained a female sterile
phenotype when I drive the UAS-IR construct using actin and tubulin GAL4
drivers. I am not sure yet whether the effect is specific to RNAi of my gene
of interest or whether it is a more non-specific effect relating to high level
overexpression of non-coding RNA. Has anyone ever heard of overexpession of
RNAi (or any other non-coding RNA) causig a non-specific female sterility?