Dear fly folks,
We are embarking on a local P hop and would like to use pooled
embryos as a source of DNA to identify mutations in the gene of interest.
I have been looking in vain for an embryonic DNA protocol. Do any of you
have a simple protocol for making DNA from pools of embryos to use in PCR?
We were thinking of starting with pools of ~50 putative "hops",
mating them to virgin females, collecting embryos (overnight?), grinding
them up, doing PCR, identifying positive pools, subdividing, and repeating
until we isolate single males with mutations (insertions, deletions) of
Any advice and protocols would be much appreciated!
Julie A. Brill, Ph. D.
The Hospital for Sick Children
Rm. 9145 Elm Wing
555 University Avenue
Toronto, ON M5G 1X8 CANADA
Phone: (416) 813-8863
Lab: (416) 813-6209
Email: jabrill at sickkids.on.ca