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Problems with anti-FLAG ab (M2 from Sigma)

Wolfgang Bottenberg bottw000 at students.uni-mainz.de
Tue Apr 23 12:32:51 EST 2002

Dear all,

i have tagged a receptor from Drosophila with the FLAG-epitope. The
monoclonal antibody M2 from Sigma
(Prod.-Nr.F3165) I wanted to use for detection is staining in the whole CNS
and not only the cells where the
tagged receptor is driven.
Did anyone have this problem with this ab too or does anybody know a better ab?
Every hint is very welcome!

Thanx in advance

Wolfgang Bottenberg

			Wolfgang Bottenberg
			Institut für Genetik- Institute of Genetics
			Becherweg 32 Raum (room) 01-141
			Johannes Gutenberg-Universität
			tel: +49 (0)6131-3925343;
			private: Mosburgstr.8; 65203 Wiesbaden
			tel: +49 (0)611-6092133
			alternative email-adress: wbottenberg at gmx.de


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