I've been trying to track the birth time of the neuronal cells using
BrdU incorporation into the larvae and the pupae of one of our Gal4
lines crossed with UAS-GFP. But after the treatment of the tissue
with 2NHCl (for the denaturation of double strands of DNA), GFP fades
Can anyone give me an advice for the alternatives (other protocols,
substitute for BrdU, etc.)?
I'd appriciate your help!
Insitute of Zoology,
University of Fribourg