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positions available

Dr. Julie Brill jbrill at sickkids.ca
Fri Dec 21 13:06:01 EST 2001

Dear members of the Drosophila community,

	I currently have two positions available in my laboratory, one for
a Research Technologist II and the other for a Postdoctoral Fellow.  I am
including summaries of the positions and potential research projects below.
I would be delighted to discuss these further with any interested
applicants.  Please bring this notice to the attention of any
well-qualified people you know.

	Best wishes for a happy holiday,

				Julie Brill
Julie A. Brill, Ph. D.

Research Institute
The Hospital for Sick Children
Rm. 9145 Elm Wing
555 University Avenue
Toronto, ON  M5G 1X8  CANADA

Phone:  (416) 813-8863
Lab:  (416) 813-6209
Email:  jbrill at sickkids.on.ca

Research Technologist II

Description of the Position:     Working with a starting investigator in a
new laboratory in the Developmental Biology program, you will carry out
experiments addressing the role of key regulatory membrane phospholipids in
cell division in the fruit fly, Drosophila melanogaster.
For this project, you will use some or all of the following techniques:
- Molecular biology (PCR, plasmid construction, RT-PCR, Northern and
Southern blots)
- Protein biochemistry (Western blots, immunoprecipitation, affinity
- Drosophila molecular genetics (genetic crosses, production of transgenic
flies, RNA in situ hybridization)
- Tissue culture (Drosophila cells)
- Cytology (phase microscopy, immunofluorescence)
- Yeast molecular biology (two-hybrid analysis)

Your duties will include: keeping the lab in smooth running order by
organizing lab stock collections (Drosophila, bacteria, yeast, tissue
culture cells, antibodies, plasmids, chemicals), maintaining Drosophila
stocks, placing lab orders, performing radiation surveys, and providing
initial training for new members of the laboratory.
The ability to maintain an independent research project would be a definite

Qualifications:     You are a highly motivated person interested in
learning new techniques.  You are well organized and possess excellent
interpersonal skills.  You are able to work independently as well as in a
Ideally, your training will include a completed B.Sc. or M.Sc. (preferred)
in biochemistry, molecular biology, genetics or a related discipline,
combined with a minimum of one year experience in a similar setting.

Hours :	35 hours per week
Salary:	Commensurate with experience
Available immediately to: 	Internal and external candidates
Deadline:	January 15, 2002

Submit Resume to :	Erin O'Hare
Human Resources, Room 5426
555 University Avenue, Toronto, Ontario
M5G 1X8

Fax:      (416) 813-5671
E-mail:  hr.recruiter at sickkids.on.ca

Postdoctoral Position

	A postdoctoral position is available in the Developmental Biology
Program at The Hospital for Sick Children.  Candidates with a strong
background in Drosophila genetics, molecular and cell biology, and/or
protein biochemistry are encouraged to apply.  Possible projects will focus
on: (a) the role of phosphoinositol lipids in cytokinesis and development
(Brill, et al. (2000) Development 127: 3855-3864); and (b) developmental
regulation of cytokinesis and intercellular bridge formation in the
Drosophila male germ line (Hime, Brill and Fuller (1996) J. Cell Sci. 109:

	The approaches we are taking include:  (1) time-lapse imaging of
developing male germ cells expressing GFP fusion proteins; (2)
structure/function analysis, biochemistry and identification of binding
partners of the Fwd phosphatidylinositol 4-kinase (PI4K); (3) reverse
genetics (P element mutagenesis, RNAi) on other Drosophila PI4Ks; (4)
genetic screens to identify targets of regulation by phosphoinositol
lipids; (5) biochemical purification and identification of components of
male germ line ring canals.

	The Hospital for Sick Children has excellent research facilities,
with access to many seminar series featuring internationally renowned
speakers.  Drosophila researchers benefit from participation in an active
cross-campus developmental biology community, as well as biweekly meetings
of a Drosophila group comprising 11 laboratories affiliated with the
University of Toronto.

	Please send curriculum vitae and names of three referees to: Dr.
Julie Brill, The Hospital for Sick Children, 555 University Avenue,
Toronto, Ontario M5G 1X8, CANADA; email jbrill at sickkids.ca.


Julie A. Brill, Ph. D.

Research Institute
The Hospital for Sick Children
Rm. 9145 Elm Wing
555 University Avenue
Toronto, ON  M5G 1X8  CANADA

Phone:  (416) 813-8863
Lab:  (416) 813-6209
Email:  jbrill at sickkids.on.ca

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