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Protein extraction protocol of IP

siau min fung siauminf at uic.edu
Fri Dec 7 13:02:52 EST 2001

HI there!

I have been trying to get protein extract from embryos for
immunoprecipitaton. I have tried some of the published protocol, such as
embryo extract buffer, PBS with triton-X. Then, homogenized embryos with a
pestle. I have not been able to get "WHOLE EMBRYOS LYSATE"!

I need HELP!! If you have a protocol or any SUGGESTIONS. PLEASE HELP!




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