I need to amplify a large DNA fragment about 20kbp, so to obtain that I
have tried to use PCR and amplify this fragment using taqplus but it
doesn't work that well.
I tried to amplify two 10kbp fragments instead and ligate it...but as my
fragments are two large the ligation doesn't work either....shall I try
cloning instead and what king of vectors should I use for cloning of such
Would be grateful for any help