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polyA detection in Drosophila cell cultures

Joao Rodrigues Joao.Rodrigues at EMBL-Heidelberg.de
Wed Nov 15 17:00:57 EST 2000


I am currently trying to detect polyA RNA in a Drosophila S2 cell line
using oligo-dT probes.

Does anyone have a good protocol for polyA detection  in cell lines
using oligos, or any other probes?
If so, what is the pattern of localisation of polyA in Drosophila cells?

Thank you.

Joao Rodrigues
(mail to: rodrigues_jp at hotmail.com)


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