I would be very grateful if someone could help me in microinjection
needle pulling with Sutter P-97. The parameters
01 Heat=700 Pull=20 Vel=18 Time=80
02 Heat=640 Pull=100 Vel=15 Time=? (not listed)
which I used successfully on P-80PC burns the filament trying to
use capillaries (Sutter):
borosilicate with filament
o.d.: 1.0mm, I.D.:0.50mm
10 cm length
What kind of filament/glass should I try to use???
Many thanks in advance!
Hungarian Academy of Sciences
Biological Research Center,
Institute of Genetics
email: Sipos at nucleus.szbk.u-szeged.hu