Sven Pfeiffer wrote:
> Hi there,
>> has anyone done Western blots with a GFP antibody from embryos? My
> own efforts are rather poor, lots of unspecific bands it seems. I am
> looking for a clean antibody and a reliable protocol.
You are not alone. A colleague in my lab tried to get an GFP-antibody to work
for detecting GFP in embryos, but that didn't work and so she resorted to in
situs. Can't help you...just wanted to let you know that I think it's a
general problem and specific to your lab.