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Inverse PCR to UAS insert lines

Ho Yin Chan chanhy at sas.upenn.edu
Fri Oct 22 19:23:25 EST 1999


Hi there,

I'm interested in doing inverse PCR to UAS P insert lines. I would like to
ask whether any of you has ever adapted the inverse PCR protocol for EP,
PlacW inserts to UAS insert lines? If so, I would be grateful if you could
let me know of the details of your protocol e.g. primer sequences, PCR
condition...

Thank you very much for your help in advance.

Best wishes,

Edwin Chan




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