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Tips on how to plasmid rescue p{PZ}?

ze cheng ze_cheng at HOTMAIL.COM
Wed Jun 23 19:36:23 EST 1999

    I am a graduate student of SUNYAB.My research project involves plasmid 
rescueing p{PZ} from p-element containing stains.I tried several times in 
vain so far,even with the combination of digestion with XbaI and NheI or 
XbaI with SpeI.The problem here is that I have to clone a very big 
plasmid(at least 7Kb).Has anyone succeeded in doing that?Can someone give me 
some tips on how to ligate and transform effiently?Thank you very much.
   My address is:ze_cheng at hotmail.com
   Does anyone know how to subscribe to the newsgroup?

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