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Precise excision

Chris Jones & Deirdre Sumpter 4christopher.jones at nashville.com
Tue Jun 22 21:03:31 EST 1999


In article <377002C6.B01312E5 at umich.edu>, Alex Nikitin <nikitin at umich.edu>
wrote:

>How do you determine the precise
> excision? Is this determined solely by looking at phenotype or there are
> other ways to tell? To put it in other words, could you direct me to a
> precise P-element exision protocol?

The *only* way to be completely certain that your excision is precise is
to sequence the revertant's DNA and show it has reverted to the parental
sequence.

Chris Jones

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