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Vesco Miloushev 09Jul99 stock recommendation

Kevin Cook kcook at bio.indiana.edu
Fri Jul 16 16:54:46 EST 1999


Does anyone know of a recessive phenotypically maked D.mel stock, that
has a X-linked suppressor of a Y-linked dominant lethal, that is
suitable for mutagenesis. If that is inexistant, could someone recommend
a stock, or two different stocks, that when mated produce inviable
males, but completely normal females (phenotypically and genotypically).
I basically want to mutagenize males, mated them, and kill of the male
F1s, while getting females that carry induced mutations, but are
otherwise normal. I greatly appreciate your advice.



You could use a T(Y;A) chromosome with a dominant temperature-sensitive
lethal on one of the translocation segregants.  We have two such stocks:

1482	T(Y;3)Antp[Ns-rv3], l(3)DTS4[1] Antp[Ns-rv3]/+
3164	T(Y;2)CyO, l(2)DTS513[1]/In(2LR)Gla, wg[Gla-1]

Mutagenizing males from these stocks, mating them to normal females and
applying the appropriate temperature treatment will result in female-only
cultures.  The female progeny from crosses with T(Y;3)Antp[Ns-rv3],
l(3)DTS4[1] Antp[Ns-rv3]/+ males will be wild type.  The female progeny
from crosses with T(Y;2)CyO, l(2)DTS513[1]/In(2LR)Gla, wg[Gla-1] will be
+/In(2LR)Gla.  (You could use T(Y;2)CyO, l(2)DTS513[1] to produce wild type
females if you first outcross stock 3164 to a wild type stock to create a
T(Y;2)CyO, l(2)DTS513[1]/+ stock for mutagenesis.)

Here is another approach that is not exactly what you described, but it is
close. You could use a stock similar to 

3969	R(YL)/C(1;YS)1.

When R(YL)/C(1;YS)1 males are crossed to normal females, all the male
progeny are sterile.  The X chromosome has the short arm of the Y appended,
but is otherwise normal.  The appended YS doesn't affect most processes and
 can be removed easily by recombination. 

We have a variety of stocks similar to 3969 including:

703	C(1;YS)1, Df(1)y-ac, w[a] ct[6] f[1]/Dp(1;YL)sc[S1]/C(1)DX, y[1] f[1]
710	R(YL)/C(1;YS)1, y[1] w[1]
1392	C(1;YS)6, In(1)EN2, oc[1] ptg[1] f[1]/R(YL)/C(1)RM, y[2] su(w[a])[1] w[a]
2546	C(1;YS)3, B[S], y[1] cv[1] v[1] f[1]/R(YL) & C(1)RM, y[1] v[1] f[1]/R(YL)
2560	C(1;YS)3, y[1] m[1] f[1] car[1]/R(YL)bb[+]
2562	C(1;YL)1, bb[+] ac[+] y[+]/C(1;YS)1, y[1] w[1] & C(1)DX, y[1] f[1]/0
3934	C(1)RM, y[2] su(w[a])[1] w[a] bb[-]/R(YL)/C(1;YS)6, In(1)EN2, oc[1]
ptg[1] f[1]
3959	C(1)RM, sc[1] v[1] f[1]/R(YL)/C(1;YS)6, In(1)EN2, w[1] oc[1] ptg[1] f[1]
3970	R(YL)/C(1;YS)1, w[1]
4093	C(1)RM, In(1)dl-49, y[1] ct[l] sn[X2]: y[1] ct[n] oc[1] ptg[1]
car[1]/R(YL)/C(1;YS)1, oc[1] ptg[1]
4452	C(1;YS)1, In(1)dl-49, y[1] v[Of] f[1]/Dp(1;YL)sc[S1]

Hope this helps,

Kevin Cook, Ph.D.		Bloomington Drosophila Stock Center
Department of Biology		http://flystocks.bio.indiana.edu
Jordan Hall 142		
Indiana University		812-855-5782
1001 E. Third St.		812-855-2577 (fax)
Bloomington, IN  47405-3700	kcook at bio.indiana.edu

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