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kc and Schneider cell lines

Hinnerk Boriss biohb at biology.au.dk
Tue Jul 6 14:23:06 EST 1999

David, many thanks for your literature suggestions!

After some reading I have another question. Both the kc and Schneider
cell lines seem to have been established in the early seventies. Do the
(commercially) available cell lines all originate from those very cell lines, or
are they recent isolates of similar cells?
Further, if they have been maintained for roughly 30 years in culture (or were
they mostly stored in the freezer?), to what extend is their mutational load


David wrote:

> There is a book devoted to the subject, called 'Drosophila Cells in
> Culture', by Guy Echalier (Academic Press, 1997). Also, there is a chapter
> in a Meth. Cell Biol. of recent years, by Cherbas (perhaps with other
> authors, I'm not sure) on this subject (you should be able to find it via
> Pubmed/Medline searches). And there is a (shorter) chapter by her in
> 'Drosophila: a practical approach', 2nd edition (1998, Oxford Univ.
> Press).
> Also, Invitrogen sells a kit for using Drosophila cells (for production of
> recombinant proteins, I *think*) - see their WWW site (probably
> www.invitrogen.com).
> Regards,
> David

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