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1. RNA isolation 2. DIG northern blot

Martin Hoehne marthoe at filab.biologie.uni-freiburg.de
Thu Feb 25 13:02:14 EST 1999

Hello everybody,

i have two questions concerning Drosophila northern blots:

1. Has anyone ever used the Qiagen RNeasy Kit to isolate Drosophila
total RNA? When I check
the RNA on a denaturing agarose-gel i only see one very prominent band
instead of the two rRNA
bands i'm expecting? The RNA does not look degraded and works very well
for RT-PCR.
Any ideas what happend with the rRNA?

2. Has anyone ever used a DIG labeled cDNA Probe for northern blots?
If so, at which concentration did you use it and what kind of
hybridization solution did you use?

Thanks in advance, Martin

Martin Hoehne
Lab Prof. Fischbach
University Freiburg, Germany

marthoe at whisky.biologie.uni-freiburg.de

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