I'm experiencing problems with hybridizations of biotinylated probes to
Drosophila polytene chromomosomes. After development of the signal (I'm
using hrp-conjugated streptavidin and DAB)and Giemsa staining, I get
very poor banding patterns on the chromsomes. I've tried lengthening the
staining time with no effect. The probe signal is fine.
Has anyone experienced this problem before and, if so, have any advice?
Thanks in advance!!
msw2002 at mail.med.cornell.edu