Hi fly people,
I want to mobilize a plArB(ry+) element 30 Kb away from its original
insertion site. I am planning to use IPCR as a screening method. Before
i set up the experiment, i hope to have some ideas about a hopping
frequency and a success rate of IPCR. Would you please share your
experience on this? Although P-factor mobilization is sensitive to its
insertion site and the nature of P-elements, it would be quite helpful to
have some stats in hands.
I greatly appreciate your input. Hope to hear many responses.