I'm looking at the expression of a gene within the midgut epithelium
and its potential regulation by homeotic genes. I did an in situ
using late stage embryos derived from abdA[M1]/TM1, Me parents. I've
gotten some odd results that I'm not sure how to interpret. One of my
problems is that I'm not certain how to identify abdA[M1] homozygous
embryos. I know that abdA mutants lack the second and third midgut
constrictions, and I can identify mutants at that particular stage.
What do older or younger mutants look like? Are there any unambiguous
features that can be used to identify abdA mutants from wild-type
embryos in whole mount in situs? Are mutant embryos very similar to
wild-type or are they wildly abnormal? Do TM1 homozygotes have a
distinctive mutant phenotype, or do they resemble wild-type embryos?
I would appreciate any information you can give me.
Department of Zoology
Oxford, OH 45056
email: kovalige at muohio.edu