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Immuno on embryos

Martin Cann mjcann at MED.CORNELL.EDU
Tue Apr 21 08:47:42 EST 1998


I have been doing immuno on late stage embryos using Vectastain and
DAB/Ni2+.  My no primery/no secondary control is giving me horrible
background (as is everything else!).  Do people find it necessary to
preabsorb the vectastain reagent or am I just not blocking sufficiently?

Any help would be appreciated.

All the best

Martin Cann

Cornell University Medical College
mjcann at med.cornell.edu



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