I have been doing immuno on late stage embryos using Vectastain and
DAB/Ni2+. My no primery/no secondary control is giving me horrible
background (as is everything else!). Do people find it necessary to
preabsorb the vectastain reagent or am I just not blocking sufficiently?
Any help would be appreciated.
All the best
Cornell University Medical College
mjcann at med.cornell.edu