IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

X-gal staining and antibodies/in situ hybs

Denise Clark clarkd at unb.ca
Mon Nov 17 15:23:34 EST 1997


A bunch of questions:
We are using a blue balancer to identify embryos that are deficiency
homozygotes.  We would like to be able to take the non-Xgal-staining embryos
and perform whole mount (1) in situ hybridization using riboprobes and (2)
antibody staining.

Are there protocols for either of these that are compatible with any X-gal
staining procedure for lacZ?  Can anyone recommend a paper where this has
been done?

A related question:  The answer is probably no, but is there a way to stain
embryos for beta-gal activity so that they live a little longer?

Also, is there a good tool/trick for handling embryos while sorting the
white from blue-staining ones?

Any tips would be appreciated,
Denise Clark
Dept. of Biology
UNB Fredericton
NB, Canada
clarkd at unb.ca







More information about the Dros mailing list

Send comments to us at biosci-help [At] net.bio.net