A bunch of questions:
We are using a blue balancer to identify embryos that are deficiency
homozygotes. We would like to be able to take the non-Xgal-staining embryos
and perform whole mount (1) in situ hybridization using riboprobes and (2)
Are there protocols for either of these that are compatible with any X-gal
staining procedure for lacZ? Can anyone recommend a paper where this has
A related question: The answer is probably no, but is there a way to stain
embryos for beta-gal activity so that they live a little longer?
Also, is there a good tool/trick for handling embryos while sorting the
white from blue-staining ones?
Any tips would be appreciated,
Dept. of Biology
clarkd at unb.ca