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Tamas, Lukacsovich lukacs at fly.erato.jst-c.go.jp
Tue Jan 21 06:04:52 EST 1997

Dear Drosophilists,

I downloaded the sequence of pUChsneo (it's synonym name is pP{hsneo})
vector from FlyBase about half a year ago. I was surprised to learn that
this vector contains a heat shock promoter driven bacterial ribosomal RNA
gene to confer neomycin resistance. (at least the given sequence for
neomycin resistance gene showed 100% homology with a piece of E. coli 16S
ribosomal RNA gene.)
It was even a bigger surprise when I checked the updated vector sequence in
Flybase a week ago and found it having changed. Now it contains the
neomycin phosphotransferase gene instead (and in fact this is really the
case as I checked by sequencing). The other part of the vector remained
I would like to know whether this confusion came from a simple submission
error or that former version of the vector is really existing and available
(I am not simply curious but rather particularly interested in special
cases of RNA mediated regulatory processes.)
Any information would be greatly appreciated.
Thanks in advance,

Tamas Lukacsovich.

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