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embryo sectioning

Kathleen Gajewski sa08383 at ODIN.MDACC.TMC.EDU
Wed Jan 8 16:33:16 EST 1997

I received an e-mail request from Srikanth Rajagopalan about embryo 
sectioning and tried to reply, but my message bounced about in cyberia 
for a week and was returned, so I'm posting it here:

Date: Tue, 31 Dec 1996 14:12:05 +0530 (IST)
To: Kathleen Gajewski <sa08383 at ODIN.MDACC.TMC.EDU>
Subject: Re: embryo sectioning

Dear Kathleen,
I've been trying to section fly ovaries with variable success. I was
wondering if you could share any useful information you may have received
about Araldite sectioning.

   National Centre for Biological Sciences
   T.I.F.R. Centre
   P.O. Box 1234                        Phone  :  (Off)3344062,(Res)5474816
   I.I.Sc. Campus                       Fax    :  080-3343851
   Bangalore - 560043                   e-mail :  snoopy at ncbs.tifrbng.res.in

On 10 Jun 1996, Kathleen Gajewski wrote:

> To any and all fly people,
>       Does any one have a detailed protocol for Araldite sectioning
> of embryos?  Is there any advantage to this method over
> doing parafin sections? Any information would be greatly appreciated!!
> Anyone with answers can reach me by e-mail at sa08383 at odin.mdacc.tmc.edu
> (or kgajewski at odin.mdacc. tmc.edu).
>                           Thanks!
>                             Kathleen Gajewski
>                           Schulz Lab
>                           Dept. of Biochem. & Molec. Bio.
>                           M D Anderson Cancer Center

Subject: Re: embryo sectioning
To: snoopy at ncbs.tifrbng.res.in (Srikanth Rajagopalan)
Date: Tue, 31 Dec 1996 12:07:33 -0600 (CST)

Sorry to say, I didn't get any response to my query.  There is a paper
with M. Leptin as one of the authors that has some gorgeous embryo
sections.  I believe it's in Development, but I can't locate my copy
right now.  That's where I would start looking for info.  We decided not
to do sections, so I haven't followed up on any of this.

                        Good luck,  Kathleen

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