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Help with in situ hybridisation.

Jose M Chaverri jmc14009 at PEGASUS.CC.UCF.EDU
Thu Feb 20 15:07:25 EST 1997

To whoever is out there doing in situ Hybridisation on drosophila embryos
(whole mount), using asymetric PCR.  I have problems with the signal (it
is weak), and i have tried everything.  I get good yields after PCR, but
when I proceed with the in situ, the signal is not as strong as expected.
Am i asking for too much?......
sincerely yours

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