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plasmid rescue from both sides of PZ

Marc Champagne M.Champagne at nospam.cellbio.duke.edu
Tue Aug 26 07:31:32 EST 1997

In article <v01540b02b025ef4480d9@[]>, liebl at CC.DENISON.EDU
(Eric C. Liebl) wrote:

> Does anyone know of a restriction enzyme combination that will allow me to
> rescue genomic DNA from the "rosy gene side" of a PZ insert?  In
> otherwords, is there a way to rescue genomic DNA on the opposite side of
> that rescued by XbaI cutting?
> Thanks, ERIC

I got a protocol off of Flybase doing exactly that.  Look for PZ at

Mon pays ce n'est pas un pays, c'est l'hiver (Vigneault).

Pls remove -nospam- from my address if you reply by e-mail

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