Subject: Time:3:03 PM
OFFICE MEMO Yeast expression library wanted Date:9/22/96
This is a rather specific request - does anybody out there have a
Drosophila cDNA library (any stage, although embryonic may be preferable)
in a yeast expression vector which they feel free to share? The library
needs to have the following specifications
(i) It must not be fused to an activation domain (like standard 2 hybrid
(ii) Expression must not be under the control of a Gal4 requiring promoter
(iii) Selectable marker should not be Ura3.
I would also be interested to know if anyone has a randomed primed fly cDNA
library suitable for a yeast-2-hybrid/interaction trap screen.
Thanks in advance