I have problems with the transfection of Schneider cells.
Using the Calciumphosphat-Coprecipitation (diNocera and Dawid) method I could
detect only few amounts of the RNAs of the transfected DNA by
S1-Nuclease-analysis in contrast to human HeLa cell transfection.
Have you got any ideas or any tricks ?
AG Benecke, Ruhr-Uni-Bochum