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eclosion problems

Kathleen Gajewski sa08383 at ODIN.MDACC.TMC.EDU
Wed Nov 20 19:27:39 EST 1996

To any interested fly people:
Since I have received a request for all the responses I got on the 
eclosion problems query, here they are.  An additional note for those 
with mutant strains; there are alleles of the Ecdysoneless locus that 
have eclosion problems (I donUt know if they are caught in any white, 
fibrous tissue though).  I have seen 3 papers on this mutant: Devel. 
Genetics (1993) 14:369-377, J. Insect Physiol (1981) v27#12, 829-837, & 
Invert. Reprod. & Dev. (1990)
17:2, 103-110.  Thank you to all that responded.

Kathleen - I'm interested in your problem because one of my strains is
also often arrested in mideclosion.  I don't see the white fibrous stuff
you describe  though.  Is it all your flies, or only 1 strain?  Could
it be a chitinase defect?

Subject: Re: eclosion problems
To: Dushay.1 at nd.edu (Mitch Dushay)
Date: Wed, 6 Nov 1996 14:58:36 -0600 (CST)

Hi Mitch,
        I'm afraid I can't be of much help to you; while I have seen this
eclosion problem in more then one strain of flies, I mostly observe it in
yw[67c23].  I don't think yw flies are any more susceptible than any
other line, but I have been sorting through huge quantities in the past
year (lots of transgenics to make) and while the problem is relatively
rare, if you look at enough flies it will turn up just often enough to
catch your attention.  The white fibrous stuff is always there.  Your fly
strain sounds interesting though.  Have you tried helping the flies out of
their pupal cases to try and see where the defect lies, i.e., do the flies
have this problem because of some muscular or neurological defect, or is
the pupal case (or moulting process) the problem?  It is delicate work,
but it can be done.   Good Luck,    Kathleen

Date: Wed, 6 Nov 1996 08:29:11 -0800
To: sa08383 at ODIN.MDACC.TMC.EDU (Kathleen Gajewski)
Subject: Re: eclosion problems

Hi Kathleen,
        Yes, it is most likely the pupal cuticle. Early on the cuticle is
almost entirely invisible but as the animal nears eclosion it reabsorbs 
all of the fluid between the pupal and adult cuticles- at this point the 
cuticle becomes fibrous and white. The strongest attatchment of adult to 
pupal cuticle is at the posterior/dorsal tip of the abdomen and I would 
guess that this is where your flies are getting hung up (or perhaps they 
aren't getting their legs free).
                James Baker
                Dept. of Zoology
                University of Washington
                Seattle, WA

To: "sa08383 at ODIN.MDACC.TMC.EDU" <sa08383 at ODIN.MDACC.TMC.EDU>
Subject: RE: eclosion problems
Date: Tue, 5 Nov 1996 17:50:00 -0600
Encoding: 48 TEXT

Dear Kathleen,

In my lab we have seen the same white fibrous material trapping pharate
adults trying to eclose.  We found very high levels of these flies in
mutants we were characterizing to isolate lethals for the Lsp-2 (Larval
serum protein-2) gene.  Like you, I have suspected that it is the pupal
cuticle that the adults are unable to disentangle themselves from.  Is
it that they are too weak?  Lack of energy, sick?  Is there some
lubricating material missing or defective or just at too low levels,
between the pupal cuticle and the adult cuticle deposited inside the
pupal case?  I have asked myself all these questions, and have not been
able to take the time and creative thought (and funding!) to figure it
out.  We are especially interested since this phenotype is
characteristic of late pupal lethals we have characterized in our Lsp
mutagenesis projects.  Since we work with serum/storage proteins whose
amino acids apparently end up being used for all adult protein
synthesis, I have had to think about many, many different possible
explanations, from amino acid utilization for ATP synthesis to specific
proteins (possibly including Lsp-2) involved in the eclosion process.

This is all to tell you that really I know no more than you are guessing
already.  However, I am very interested in any answers you may get.
Would you mind sharing them with me?  I would be very grateful.

Thanks for being observant and willing to share your thoughts with other


Date: Wed, 6 Nov 96 16:32:19 EST
To: sa08383 at ODIN.MDACC.TMC.EDU
Subject: Re: eclosion problems


I always assumed this was peripodial membrane.  Could be wrong of course ...

--  Kathy

Kathy Matthews                  Bloomington Drosophila Stock Center

To: sa08383 at odin.mdacc.tmc.edu
Subject: Re: eclosion problems
X-Newsreader: NN version 6.5.0 #3 (NOV)
Date: Mon, 11 Nov 96 16:08:14 GMT

Dear Kathleen:
Could you please also send me the information you collected about the
eclosion problems? We have a line which has higher percentage of
eclosion failure, and I don't know the reason. Thanks in advance for
your help,


Dear Kathleen,
did you get any responses for this query?  I would be interested in the
information you collected.  Perhaps you could post a summary?

I don't know what those tissue are but I do know that some circadian
rhythm mutations affects eclosion (e.g. timeless, lark)

Tommy Wang
sci40743 at nus.sg

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