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staining embryos

mjcann at MED.CORNELL.EDU mjcann at MED.CORNELL.EDU
Fri Nov 8 20:59:26 EST 1996


I am currently trying to label a message on 0-4hr CS embryos using a DIG 
labelled riboprobe.  My problem is that, despite the fact that my 
positive control works fine and that my transcript is abundant, I 
repeatedly see no staining. Can anyone please suggest avenues by which I 
can try to improve my protocol with respect to the size of riboprobe 
(with or without alkaline hydrolysis), time of prehyb and probing, 
temperature, fixation, etc., as I am making little progress in 
elucidating the expression pattern of my transcript.

Many thanks for your help.

E-mail me directly or to the group.

Martin Cann
mjcann at med.cornell.edu






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